《植物生理学报》 2010, 46(9): 871-875

两种新型目标分子标记技术——CDDP 与PAAP

熊发前¹, 蒋菁¹, 钟瑞春¹, 韩柱强¹, 贺梁琼¹, 李忠¹, 庄伟建^3,*, 唐荣华^1,2,*

1 广西农业科学院经济作物研究所, 南宁530007; 2 广西作物遗传改良生物技术重点开放实验室, 南宁530007; 3 福建农林大学作物科学学院, 福州350002

通信作者：庄伟建；E-mail: tronghua@163.com, zhuangwj@pub2.fz.fj.cn；Tel: 0771-3276055

摘　要：

本文介绍了CDDP 和PAAP 这两种新型目标分子标记技术的产生原理、引物设计、PCR 扩增、PCR产物检测手段、分子标记特征及带型模式, 在此基础上对它们的技术特点和技术优势进行了概括, 最后, 对它们的应用前景进行了展望。

关键词：来源保守DNA序列多态性; 启动子锚定扩增多态性; 目标分子标记; DNA保守序列; 启动子

收稿：2010-04-27   修定：2010-07-26

资助：国家自然科学基金(30660094)、国家高技术研究发展计划(“863” 计划) (AA10A104)、广西科学基金(桂科自0832088)和广西科技攻关项目(桂科攻0815008-1-4)。

Two Novel Targeted Molecular Marker Techniques—CDDP and PAAP

XIONG Fa-Qian¹, JIANG Jing¹, ZHONG Rui-Chun¹, HAN Zhu-Qiang¹, HE Liang-Qiong¹, LI Zhong¹, ZHUANG Wei-Jian^3,*,TANG Rong-Hua^1,2,*

¹Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; ²Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; ³College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Corresponding author: ZHUANG Wei-Jian; E-mail: tronghua@163.com, zhuangwj@pub2.fz.fj.cn; Tel: 0771-3276055

Abstract:

This article describes principles, primer design, PCR amplification, detection methods of PCR products, characteristics of molecular markers and band patterns of two types of targeted molecular marker techniques (CDDP and PAAP). Then their technical features and technical advantages were summarized. Finally, their applications in the future were discussed.

Key words: conserved DNA-derived polymorphism; promoter anchored amplified polymorphism; targeted molecular marker; DNA conserved sequence; promoter